Document 0577
 DOCN  M9460577
 TI    Single amino acid substitution (58Pro-->Ser) in HTLV-I tax results in
       loss of ras cooperative focus formation in rat embryo fibroblasts.
 DT    9408
 AU    Matsumoto K; Akashi K; Shibata H; Yutsudo M; Hakura A; Osaka Red Cross
       Blood Center, Japan.
 SO    Virology. 1994 May 1;200(2):813-5. Unique Identifier : AIDSLINE
       MED/94233748
 AB    Two tax genes cloned from a healthy HTLV-I carrier in whom the viral
       genome is clonally integrated into peripheral CD4+CD8+ cells showed a
       considerable difference in ras cooperative focus forming ability in rat
       embryo fibroblasts (REF). Sequence analysis revealed differences in two
       codons of the two genes. SH-1tax and SH-2tax. Studies using recombinants
       between these two tax genes showed that the deficiency in ability of
       SH-1tax to cooperate with ras in focus formation in REF was caused by a
       58Pro-->Ser substitution. This amino acid substitution did not affect
       other tax functions such as colony formation in soft agar, focus
       formation in Rat-1 cells, immortalization of REF, and transcriptional
       activation through the CREB/ATF and NFkB/rel pathways. These results
       suggest that the domain of tax required for cooperative focus formation
       with ras in primary rat fibroblasts may be different from those required
       for other tax functions.
 DE    Animal  Case Report  Cell Transformation, Viral/*GENETICS  Cloning,
       Molecular  Fibroblasts  Gene Products, tax/*GENETICS  Genes,
       ras/*GENETICS  Human  HTLV-I/*GROWTH & DEVELOPMENT/GENETICS  HTLV-I
       Infections/*MICROBIOLOGY  Mutation  Plaque Assay  Proline/GENETICS  Rats
       T-Lymphocyte Subsets/MICROBIOLOGY  Trans-Activation (Genetics)  Virus
       Integration  JOURNAL ARTICLE

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